Live Imaging of mRNA Transcription in Drosophila Embryos Journal Articles

abstract

• Live imaging has been used in recent years for the understanding of dynamic processes in biology, such as embryo development. This was made possible by a combination of advancements in microscopy, leading to improved signal-to-noise ratios and better spatial and temporal resolutions, and by the development of new fluorescence markers, allowing for the quantification of protein expression and transcriptional dynamics in vivo. Here we describe a general protocol, which can be used in standard confocal microscopes to image early Drosophila melanogaster embryos, in order to learn about the transcriptional dynamics of a fluorescently labeled RNA.

authors

• Perez-Romero, Carmina Angelica
• Tran, Huy
• Coppey, Mathieu
• Walczak, Aleksandra M
• Fradin, Cecile
• Dostatni, Nathalie

status

• published 

publication date

• 2018

has subject area

• 0399 Other Chemical Sciences (FoR)
• 0601 Biochemistry and Cell Biology (FoR)
• Animals (MeSH)
• Developmental Biology (Science Metrix)
• Drosophila melanogaster (MeSH)
• Embryo, Nonmammalian (MeSH)
• Female (MeSH)
• Image Processing, Computer-Assisted (MeSH)
• Male (MeSH)
• Microscopy, Confocal (MeSH)
• RNA, Messenger (MeSH)
• Transcription, Genetic (MeSH)

published in

• Methods in molecular biology (Clifton, N.J.)  Journal

keywords

• Animals
• Confocal microscopy
• Drosophila melanogaster
• Embryo
• Embryo, Nonmammalian
• Female
• Image Processing, Computer-Assisted
• Live imaging
• MS2 system
• Male
• Microscopy, Confocal
• RNA
• RNA, Messenger
• Transcription, Genetic

Digital Object Identifier (DOI)

• 10.1007/978-1-4939-8772-6_10

PubMed ID

• 30324598

start page

• 165

end page

• 182

volume

• 1863