Differential staining of different types of peripheral nerve and muscle

We modified Ungewitter's (1951) and Beermann and Cassens' (1976) non-selective silver methods for peripheral nerve axons and their terminals by lengthening the time for the prestaining treatment of sections, adjusting the times of various stages of the staining procedures and selecting reagents with minimal chloride and sulphate impurities. These methods were applied to Bouin's fixed material embedded in wax with serial sections up to 100 …