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Reconstitution of rabbit sarcoplasmic reticulum...
Journal article

Reconstitution of rabbit sarcoplasmic reticulum calcium ATPase in a series of phosphatidylcholines containing a saturated and an unsaturated chain: suggestion of an optimal lipid environment

Abstract

The calcium-dependent ATPase from sarcoplasmic reticulum of rabbit has been purified and reconstituted in dispersions containing pure phosphatidylcholines. Each phosphatidylcholine (PC) had palmitate (16:0) at the sn-1 position of glycerol and stearate (18:0), oleate (18:1), linoleate (18:2), arachidonate (20:4), or docosahexaenoate (22:6) at the sn-2 position. The activities and activation energies of the enzyme indicated that the best enzyme …

Authors

Matthews PL; Bartlett E; Ananthanarayanan VS; Keough KM

Journal

Biochemistry and Cell Biology, Vol. 71, No. 7-8, pp. 381–389

Publisher

Canadian Science Publishing

Publication Date

July 1, 1993

DOI

10.1139/o93-056

ISSN

0829-8211