n‐3 Polyunsaturated fatty acid‐induced changes in the molecular species composition of diradylglycerol in murine peritoneal macrophages remain stable during incubation<i>ex vivo</i>

AbstractThree‐ to four‐week‐old C57BL/6 mice were maintained for four weeks on diets in which the 10% lipid component was the ethyl esters of either corn oil or n‐3 polyunsaturated fatty acids (n‐3 PUFA). Incubation of macrophagesex vivo for 14 h, a normal incubation time used for macrophage studies, in the absence of fetal calf serum, did not diminish the extent of incorporation of 20∶5n‐3 (eicosapentaenoic acid) and 22∶6n‐3 (docosahexaenoic acid) moieties into membrane phospholipids and into diradylglycerol (DG) relative to that after a very abbreviated incubation time. We conclude that studies examining the effects of dietary n‐3 PUFA on DG formation and related physiological effects in macrophages can be performed after a normalex vivo incubation of at least 14 h without experiencing a significant loss of incorporated n‐3 PUFA.

n‐3 Polyunsaturated fatty acid‐induced changes in the molecular species composition of diradylglycerol in murine peritoneal macrophages remain stable during incubationex vivo Journal Articles