Journal article
A Cellular Factor Binds to the Herpes Simplex Virus Type 1 Transactivator Vmw65 and Is Required for Vmw65-Dependent Protein-DNA Complex Assembly with Oct-1
Abstract
The herpes simplex virus transactivator Vmw65 assembles into a multicomponent protein-DNA complex along with the octamer binding protein Oct-1. Using affinity chromatography on columns conjugated with purified Vmw65 fusion protein expressed in Escherichia coli, we demonstrate that a cellular factor, distinct from Oct-1, binds to Vmw65 in the absence of target DNA and is necessary for Vmw65-mediated complex assembly with Oct-1.
Authors
Xiao P; Capone JP
Journal
Molecular and Cellular Biology, Vol. 10, No. 9, pp. 4974–4977
Publisher
Taylor & Francis
Publication Date
September 1, 1990
DOI
10.1128/mcb.10.9.4974-4977.1990
ISSN
0270-7306