We previously identified Xin, a novel actin binding protein, to be upregulated during the early phases of skeletal muscle regeneration. Here we demonstrate the transcriptional regulation of Xin within skeletal muscle and assess its function within satellite cell progeny (myoblasts). To define transcriptional regulation, promoter‐reporter assays were performed in the presence of known myogenic transcription factors (MEF2, MyoD and Myf‐5). All factors tested were capable of transactivating Xin promoter constructs, supporting the muscle‐specific expression of Xin. To determine the function of Xin within myoblasts, we infected C2C12 myoblasts with adenoviral Xin shRNA and assessed proliferation, migration and differentiation. Inhibition of endogenous Xin expression resulted in a 26% increase in cell proliferation (581.4 ± 7.1 vs 461.8 ± 7.2 cells (x103), counted 24h after initial 200,000 plated, n=5, p<0.05), a 20% increase in migratory capacity (288μm ± 6μm vs 240μm ± 3μm, distance traveled 24h after plate‐scratch, n=3, p<0.05), and enhanced differentiation into mature myotubes as assessed by Western blot analysis of myosin heavy chain (0.488 ± 0.04 vs 0.159 ± 0.03 RLU, n=3, p<0.05). These findings are the first to demonstrate that alterations in Xin expression modify muscle precursor cell function. Future studies will elucidate the function of Xin within these cells during skeletal muscle regeneration.