Journal article
Making AppDNA using T4 DNA ligase
Abstract
5('),5(')-Adenylyl pyrophosphoryl DNA (AppDNA) contains a high-energy pyrophosphate linkage and can be exploited as an activated DNA substrate to derive new DNA enzymes for carrying out various DNA modification reactions. For this reason, enzymatic synthesis of AppDNA is highly desirable. AppDNA is a known intermediate in DNA ligase mediated DNA ligation reactions, but rarely accumulates under normal reaction conditions. Here we report that T4 …
Authors
Chiuman W; Li Y
Journal
Bioorganic Chemistry, Vol. 30, No. 5, pp. 332–349
Publisher
Elsevier
Publication Date
October 2002
DOI
10.1016/s0045-2068(02)00018-4
ISSN
0045-2068