Journal article
Purification of bovine striatal dopamine D-2 receptor by affinity chromatography.
Abstract
Bovine striatal dopamine D-2 receptor has been purified approximately 2000-fold by affinity chromatography. The receptor, solubilized with cholic acid and sodium chloride, was adsorbed on haloperidol-linked Sepharose CL-6B and eluted with spiroperidol. The adsorption of receptor to the affinity matrix was biospecific as preincubation of the solubilized preparation with D-2 receptor agonists or antagonists blocked retention of receptor. The …
Authors
Ramwani J; Mishra RK
Journal
Journal of Biological Chemistry, Vol. 261, No. 19, pp. 8894–8898
Publisher
Elsevier
Publication Date
7 1986
DOI
10.1016/s0021-9258(19)84466-6
ISSN
0021-9258