Journal article
Reconstitution of affinity-purified dopamine D2 receptor binding activities by specific lipids
Abstract
The role of lipids in maintaining ligand binding properties of affinity-purified bovine striatal dopamine D2 receptor was investigated in detail. The receptor, purified on a haloperidol-linked Sepharose CL6B affinity column, exhibited low [3H]spiroperidol binding unless reconstituted with soybean phospholipids. In order to understand the role of individual phospholipids in maintaining the receptor binding activity, the purified preparation was …
Authors
Srivastava LK; Kazmi SMI; Blume AJ; Mishra RK
Journal
Biochimica et Biophysica Acta, Vol. 900, No. 2, pp. 175–182
Publisher
Elsevier
Publication Date
June 1987
DOI
10.1016/0005-2736(87)90331-2
ISSN
0006-3002