Quantification of sulphur amino acids by ultra-high performance liquid chromatography in aquatic invertebrates Academic Article uri icon

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abstract

  • We examined the performance of an ultra-high performance liquid chromatography method to quantify protein-bound sulphur amino acids in zooplankton. Both cysteic acid and methionine sulfone were linear from 5 to 250 pmol (r2 = 0.99), with a method detection limit of 13 pmol and 9 pmol, respectively. Although there was no matrix effect on linearity, adjacent peaks and co-eluting noise from the invertebrate proteins increased the detection limits when compared to common standards. Overall, performance characteristics were reproducible and accurate, and provide a means for quantifying sulphur amino acids in aquatic invertebrates, an understudied group.

authors

  • Thera, Jennifer C
  • Kidd, Karen
  • Dodge-Lynch, M Elaine
  • Bertolo, Robert F

publication date

  • December 2017