We investigated the prevalence of vulvovaginal candidiasis due to
C. africanain an STDclinic in India and analysed the genetic relatedness of these C. africanaisolates with those outside India. A total of 283 germ‐tube‐positive yeasts were identified by VITEK2. Molecular characterisation of all isolates was carried out by hwp1‐gene‐specific PCR. Of 283 germ‐tube‐positive yeast isolates, four were identified as C. africanausing hwp1‐gene‐specific PCR. All hwp1 PCRpositive C. africanawere subjected to antifungal susceptibility testing, ITSand D1/D2 region sequencing and were typed by using MLSTapproach. Similar to C. africanaisolates from the United Kingdom and unlike those from Africa, the Indian C. africanagrew at 42°C. Sequencing of eight gene fragments in MLSTidentified all four strains to have different genotypes not reported previously. Furthermore, though the Indian C. africanaisolates were susceptible to most of the 14 tested antifungal drugs, differences in susceptibility were observed among the four strains. Our results indicate genetic and phenotypic heterogeneity among C. africanafrom different geographical regions. Due to lack of data on epidemiology and genetic variability of this under‐reported yeast, more studies using molecular methods are warranted.