Adrenal chromaffin cell function in high‐altitude deer mice (Peromyscus maniculatus)
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abstract
Hypoxia at high altitudes can activate the sympathetic nervous system, which could become maladaptive in high‐altitude natives if it is maintained over chronic periods. The deer mouse (Peromyscus maniculatus) has the largest altitudinal range of all North American mammals, with populations living above 4300m elevation. We hypothesized that high‐altitude deer mice might have altered the physiology of chromaffin cells in the adrenal gland to avoid chronic activation of the sympathetic response. We used captive breeding colonies derived from wild populations at high and low altitudes, and compared them in normoxia and after 6–8 weeks of acclimation to hypoxia (12kPa O2, simulating the PO2 at ~4300m). High‐altitude mice show lower rates of catecholamine secretion from adrenal slices (measured via carbon fibre amperometry) in response to stimulation by a nicotinic acetylcholine receptor agonist (nicotine). Here, we used ratiometric Ca2+ imaging of cultured chromaffin cells to assess whether the variation in catecholamine secretion resulted from variation in the Ca2+ signal for vesicular catecholamine release. The rise of intracellular Ca2+ in response to nicotine (1, 5, 25, 125μM) did not vary between populations and was not affected by hypoxia acclimation, suggesting that events downstream of Ca2+ influx likely contribute to population differences in catecholamine secretion. This appeared to include differences in catecholamine stores, which were lower in highlanders compared to lowlanders in cultured cells from normoxia‐acclimated mice. Our data therefore suggest that chronic sympathetic activation may be avoided in deer mice at high altitudes by a reduction in catecholamine storage and release from the adrenal gland.
