Purification of lysozyme by microporous PVDF membrane-based chromatographic process

Microporous and macroporous membranes can be used to carry out chromatographic separation processes, thereby overcoming some of the limitations associated with packed bed columns. A commercially available polyvinylidine fluoride (PVDF) membrane was identified which could selectively bind lysozyme by a cation exchange mechanism. The PVDF membrane used was reported to be resistant to a wide variety of organic solvents, acids and bases, and could …