Journal article
Recovery and isolation of recombinant human monoclonal antibody from transgenic tobacco plants
Abstract
Early steps in the purification process for obtaining recombinant antibody from transgenic plants can significantly affect the overall purity and recovery. We investigate the effects of salt concentration, tissue grinding time and lowering pH on recovery and purification of a recombinant human monoclonal antibody (anti-Pseudomonas aeruginosa serotype O6ad) from transgenic tobacco plants. The presence of 450mM sodium chloride in the extraction …
Authors
Mayani M; McLean MD; Hall JC; Filipe CDM; Ghosh R
Journal
Biochemical Engineering Journal, Vol. 54, No. 2, pp. 103–108
Publisher
Elsevier
Publication Date
4 2011
DOI
10.1016/j.bej.2011.02.004
ISSN
1369-703X