Journal article
Instability of toxin A subunit of AB5 toxins in the bacterial periplasm caused by deficiency of their cognate B subunits
Abstract
Shiga toxin (STx) belongs to the AB(5) toxin family and is transiently localized in the periplasm before secretion into the extracellular milieu. While producing outer membrane vesicles (OMVs) containing only A subunit of the toxin (STxA), we created specific STx1B- and STx2B-deficient mutants of E. coli O157:H7. Surprisingly, STxA subunit was absent in the OMVs and periplasm of the STxB-deficient mutants. In parallel, the A subunit of …
Authors
Kim S-H; Ryu SH; Lee S-H; Lee Y-H; Lee S-R; Huh J-W; Kim S-U; Kim E; Kim S; Jon S
Journal
Biochimica et Biophysica Acta, Vol. 1808, No. 10, pp. 2359–2365
Publisher
Elsevier
Publication Date
October 2011
DOI
10.1016/j.bbamem.2011.06.016
ISSN
0006-3002