Mature induced-pluripotent-stem-cell-derived human podocytes reconstitute kidney glomerular-capillary-wall function on a chip Academic Article uri icon

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abstract

  • An in vitro model of the human kidney glomerulus - the major site of blood filtration - could facilitate drug discovery and illuminate kidney-disease mechanisms. Microfluidic organ-on-a-chip technology has been used to model the human proximal tubule, yet a kidney-glomerulus-on-a-chip has not been possible because of the lack of functional human podocytes - the cells that regulate selective permeability in the glomerulus. Here, we demonstrate an efficient (> 90%) and chemically defined method for directing the differentiation of human induced pluripotent stem (hiPS) cells into podocytes that express markers of the mature phenotype (nephrin+, WT1+, podocin+, Pax2-) and that exhibit primary and secondary foot processes. We also show that the hiPS-cell-derived podocytes produce glomerular basement-membrane collagen and recapitulate the natural tissue/tissue interface of the glomerulus, as well as the differential clearance of albumin and inulin, when co-cultured with human glomerular endothelial cells in an organ-on-a-chip microfluidic device. The glomerulus-on-a-chip also mimics adriamycin-induced albuminuria and podocyte injury. This in vitro model of human glomerular function with mature human podocytes may facilitate drug development and personalized-medicine applications.

authors

  • Didar, Tohid
  • Musah, Samira
  • Mammoto, Akiko
  • Ferrante, Thomas C
  • Jeanty, Sauveur SF
  • Hirano-Kobayashi, Mariko
  • Mammoto, Tadanori
  • Roberts, Kristen
  • Chung, Seyoon
  • Novak, Richard
  • Ingram, Miles
  • Fatanat-Didar, Tohid
  • Koshy, Sandeep
  • Weaver, James C
  • Church, George M
  • Ingber, Donald E

publication date

  • May 2017