[9] Primary structure of bacteriorhodopsin: Sequencing methods for membrane proteins

Publisher This chapter discusses the sequencing methods for membrane proteins. For sequencing, separation procedure is required. Separation of the short-sized fragments achieved by gel permeation in organic solvents using Sephadex LH-20. The corresponding problem of the separation of C-1 fragments containing larger and more hydrophobic fragments is solved by a combination of reverse-phase HPLC 1° and gel permeation using Sephadex LH-60 in …