Regulation of rat soleus muscle carnitine palmitoyl transferase-I activity in vitro

Carnitine palmitoyltransferase-I (CPT-I) is considered to be a rate-limiting step in the mitochondrial translocation and β-oxidation of free fatty acids. Based on in vitro studies, the rest and exercise concentrations of its inhibitor, malonyl-CoA (M-CoA), should significantly inhibit CPT-I activity and preclude the increase in free fatty acid use during exercise. We investigated the effects of substrate concentration, pH, temperature, and …