A low carbohydrate diet increases human skeletal muscle pyruvate dehydrogenase activity

To characterize human skeletal muscle enzymatic adaptation to a low carbohydrate (CHO) diet, subjects consumed a diet consisting of 5% CHO, 62% fat, and 33% protein for 6 d compared to their normal diet (48% CHO, 34% fat, and 18% protein). Biopsies were taken from the vastus lateralis prior to and following 3 and 6 d on a low CHO diet. Intact mitochondria were extracted from fresh muscle and were analyzed for pyruvate dehydrogenase (PDH) kinase …