Transduction of the reaction between urea and covalently immobilized urease by fluorescent amphiphilic membranes

Amphiphiles with chain lengths of 12 and 16 carbons having a triethoxy chlorosilane group at one terminus were covalently immobilized by reaction with hydroxyl groups on the surfaces of planar quartz wafers and optical fibres. The enzyme urease was covalently immobilized onto either carboxylic acid or amine functionalities at the other terminus of the immobilized amphiphiles, resulting in a surface coverage of about 60% of a close-packed …