Time-Resolved Fluorescence Anisotropy in Assessing Side-Chain and Segmental Motions in Polyamines Entrapped in Sol−Gel Derived Silica

The side-chain and backbone dynamics of two model polyamines, polylysine (PL) and poly(allylamine) (PAM), were examined with time-resolved fluorescence anisotropy (TRFA) in aqueous solution and when the polyamines were entrapped into sol−gel derived silica. Both polyamines were ionically labeled with fluorescein, causing the rotational characteristics of the probe to be interconnected to the dynamics of the polyamine chain. TRFA studies of the …