Anaplerotic flux of muscle tricarboxylic acid cycle intermediates during exercise and recovery in humans

We examined changes in tricarboxylic acid cycle intermediates (TCAI) in human skeletal muscle during 5 min of dynamic knee extensor exercise (∼80% of maximum workload) and after 2 min of recovery. The total concentration of TCAI (∑TCAI) increased (P≤0.05) from 1.10±0.08 at rest to 3.12±0.24, 3.86±0.35 and 4.33±0.30 mmol·kg^-1 dry wt (dw) after 1, 3 and 5 min of exercise, respectively. The rapid increase in the ∑TCAI was primarily mediated by …