Effect of alginate on innate immune activation of macrophages

AbstractAlginate, a natural polysaccharide, has been widely used in tissue engineering and drug delivery, butlike other biomaterials, it causes inflammation by unknown mechanisms. We hypothesized that alginate would stimulate innate immune responses through macrophage receptors. In this study, we showed that sodium alginate induced activation of macrophage‐like cells (RAW264.7) through the NF‐κB pathway. Production of proinflammatory cytokines, such as IL‐1β, IL‐6, IL‐12, and TNF‐α was time and dose‐dependent. Treatment with alginate solution caused responses that closely paralleled stimulation by lipopolysaccharide in timing and magnitude. These data suggest that sodium alginate causes innate immune responses through NF‐κB activation and likely activates the same pathways as pathogen recognition. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res, 2009

Effect of alginate on innate immune activation of macrophages Journal Articles