Improving the protein activity and stability under acidic conditions via site-specific conjugation of a pH-responsive polyelectrolyte

Maintaining the protein activity and stability under acidic conditions is important in bioengineering and biomedical applications. Polyelectrolyte conjugation as a means of stabilizing proteins has received much recent attention. Retention of protein activity, and especially, improvement of protein stability by minimizing the number of polymer chains in the conjugate, as well as by choosing the optimal site for conjugation, is critical in …