Analysis of proteins adsorbed to glass from human plasma using immunoblotting methods

Glass beads were contacted with heparinized human plasma and the adsorbed proteins eluted sequentially with 1 M tris buffer, pH 7.4, and 2% SDS. The proteins in the eluates were analyzed by SDS-polyacrylamide gel electrophoresis and immunoblotting procedures using antisera to 16 common plasma proteins. All sixteen proteins were found to be present in the eluates. The immunoblots provided evidence of contact phase activation although the role of …