Journal article
Immunoblot analysis of proteins associated with HEMA-MMA microcapsules: Human serum proteins in vitro and rat proteins following implantation
Abstract
Human serum proteins and their fragments, associated with hydroxyethyl methacrylate-methyl methacrylate (HEMA-MMA) copolymer microcapsules, were characterized using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblot analysis. Capsules were incubated with serum for 1 week in vitro and then dissolved in ethanol to also precipitate the adsorbed protein. The precipitate was dissolved in 2% (w/v) SDS (the 'capsule …
Authors
Babensee JE; Cornelius RM; Brash JL; Sefton MV
Journal
Biomaterials, Vol. 19, No. 7-9, pp. 839–849
Publisher
Elsevier
Publication Date
4 1998
DOI
10.1016/s0142-9612(97)00242-1
ISSN
0142-9612
Associated Experts
Fields of Research (FoR)
Medical Subject Headings (MeSH)
AnimalsBiocompatible MaterialsBlood ProteinsCalciumCapsulesComplement Inactivator ProteinsComplement System ProteinsCulture MediaEdetic AcidElectrophoresis, Polyacrylamide GelHot TemperatureHumansImmunoblottingMaleMethylmethacrylatesPolyhydroxyethyl MethacrylateProstheses and ImplantsRatsRats, Wistar