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Use of an improved quantitative polymerase chain...
Journal article

Use of an improved quantitative polymerase chain reaction assay to determine differences in human rhinovirus viral loads in different populations

Abstract

Human rhinoviruses (HRV) frequently cause acute respiratory infections and chronic respiratory disease exacerbations. However, testing is not generally offered. We developed a modified HRV quantitative polymerase chain reaction (qPCR) assay to assess viral loads in the community and hospital patients. The assay had a lower limit of detection of 2 log(10) viral copies/mL and displayed linearity over 5 log(10) viral copies, with a lower limit of …

Authors

Granados A; Luinstra K; Chong S; Goodall E; Banh L; Mubareka S; Smieja M; Mahony J

Journal

Diagnostic Microbiology and Infectious Disease, Vol. 74, No. 4, pp. 384–387

Publisher

Elsevier

Publication Date

December 2012

DOI

10.1016/j.diagmicrobio.2012.08.023

ISSN

0732-8893