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Characterization of viability and proliferation of...
Journal article

Characterization of viability and proliferation of alginate‐poly‐L‐lysine–alginate encapsulated myoblasts using flow cytometry

Abstract

Genetically modified cells encapsulated in alginate-poly-L-lysine-alginate (APA) are being developed to deliver therapeutic products to treat a variety of diseases. The characterization of the encapsulated cells thus becomes paramount. This study reports a novel method to assess the viability, granularity and proliferation of encapsulated cells based on flow cytometry. The in vitro viability of encapsulated G8 murine myoblasts secreting canine …

Authors

Thakur A; Sengupta R; Matsui H; Lillicrap D; Jones K; Hortelano G

Journal

Journal of Biomedical Materials Research Part B Applied Biomaterials, Vol. 94B, No. 2, pp. 296–304

Publisher

Wiley

Publication Date

August 2010

DOI

10.1002/jbm.b.31648

ISSN

1552-4973