Journal article
Integrated Solid-Phase Synthesis and Purification of PEGylated Protein
Abstract
We describe an integrated method for solid-phase protein PEGylation and the purification of mono-PEGylated protein thus synthesized. Lysozyme was used as model protein in this study. Methoxy-polyethyleneglycol propionaldehyde (or m-PEG propionaldehyde) was first immobilized on a stack of microporous hydrophobic interaction membranes housed in a module. The membrane-bound m-PEG propionaldehyde was then contacted with lysozyme solution, which …
Authors
Shang X; Yu D; Ghosh R
Journal
Biomacromolecules, Vol. 12, No. 7, pp. 2772–2779
Publisher
American Chemical Society (ACS)
Publication Date
July 11, 2011
DOI
10.1021/bm200541r
ISSN
1525-7797