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Determination of N-acetylserotonin and Melatonin...
Journal article

Determination of N-acetylserotonin and Melatonin Activities in the Pineal Gland, Retina, Harderian Gland, Brain and Serum of Rats and Chickens

Abstract

An N-acetylserotonin/melatonin radioimmunoassay (NAS/Mel RIA) and a Mel RIA were developed to measure NAS and Mel contents in tissues of rats and chickens. Anti-NAS and anti-Mel sera were produced by immunization of rabbits with NAS-M-bovine serum albumin (BSA) and Mel-M-BSA, respectively. Anti-NAS serum used in the NAS/Mel RIA reacts equally well with NAS and Mel, while anti-Mel serum used in the Mel/RIA reacts specifically with Mel. The NAS …

Authors

Pang SF; Brown GM; Grota LJ; Chambers JW; Rodman RL

Journal

Neuroendocrinology, Vol. 23, No. 1, pp. 1–13

Publisher

Karger Publishers

Publication Date

1977

DOI

10.1159/000122649

ISSN

0028-3835