Journal article
Antibodies to indolealkylamines II: site of conjugation of melatonin to protein using formaldehyde
Abstract
The site of coupling on the hapten was investigated for melatonin, formaldehyde, and protein conjugates. Model reactions of glycine ethyl ester and piperidine with formaldehyde and melatonin suggested that coupling first occurred through the indole nitrogen and then a stable bond was formed at C-2. Cross-reaction studies of antisera stimulated by melatonin, formaldehyde, and protein conjugates support the hypothesis that C-2 is the site of …
Authors
Grota LJ; Snieckus V; Silva SOD; Brown GM
Journal
Biochemistry and Cell Biology, Vol. 61, No. 10, pp. 1096–1101
Publisher
Canadian Science Publishing
Publication Date
October 1, 1983
DOI
10.1139/o83-139
ISSN
0829-8211