Journal article
Solid‐Phase Enzyme Activity Assay Utilizing an Entrapped Fluorescence‐Signaling DNA Aptamer
Abstract
Layered enzyme assays: The use of a layered sol‐gel based material allows for spatial separation of coimmobilized enzyme and fluorescent aptamer reporter species. This provides a route for facile assaying of enzyme activity and inhibition in cases where the aptamer signals the presence of the enzyme substrate. This then results in accurate IC50 determinations.
Authors
Rupcich N; Nutiu R; Li Y; Brennan JD
Journal
Angewandte Chemie International Edition, Vol. 45, No. 20, pp. 3295–3299
Publisher
Wiley
Publication Date
May 12, 2006
DOI
10.1002/anie.200504576
ISSN
1433-7851