Journal article
Calyculin A-induced endothelial cell shape changes are independent of [Ca2+]i elevation and may involve actin polymerization
Abstract
Changes in endothelial cell (EC) shape result in inter-EC gap formation and subsequently regulate transendothelial passage. In this work, we investigated the effects of protein phosphorylation (induced by inhibition of protein phosphatases) on EC shape changes. Treatment of bovine pulmonary artery endothelial cells (BPAEC) with calyculin A (100 nM, an inhibitor of protein Ser/Thr phosphatases 1 and 2A) resulted in cell retraction, surface bleb …
Authors
Leung YM; Kwan TK; Kwan CY; Daniel EE
Journal
Biochimica et Biophysica Acta, Vol. 1589, No. 2, pp. 93–103
Publisher
Elsevier
Publication Date
April 2002
DOI
10.1016/s0167-4889(02)00161-1
ISSN
0006-3002