Journal article
The proteolytic nature of commercial samples of galactose oxidase Purification of the enzyme by a simple affinity method
Abstract
Several commercially available samples of galactose oxidase (D-galactose: oxygen 6-oxidoreductase, EC 1.1.3.9) were found to contain high proteolytic activity on proteins such as fibrinogen, transferrin, albumin and casein. A simple, efficient method was devised for the purification of galactose oxidase which relies on the affinity of the enzyme for agarose (Sepharose 6B). The purified galactose oxidase was recovered in high yield free from …
Authors
Hatton MWC; Regoeczi E
Journal
Biochimica et Biophysica Acta, Vol. 438, No. 2, pp. 339–346
Publisher
Elsevier
Publication Date
7 1976
DOI
10.1016/0005-2744(76)90251-5
ISSN
0006-3002