Chromatography of Suspensions - Analytical Corrections for Axial Dispersion

An analytical method is proposed to correct the signal from a turbidity detector for axial dispersion in the chromatography of particle suspensions. An earlier analytical method due to Hamielec and Singh [1] is limited to light scattering in the Rayleigh regime. The solution reported herein is derived using the more general Mie light scattering theory and enables the correction of various moments of the particle diameter frequency distribution function. The axial dispersion phenomenon is described by the well known integral equation where the instrumental spreading function is assumed Gaussian. The solution is applicable for both linear and nonlinear particle diameter-retention volume calibration curves.