The kdsA gene of Pasteurella trehalosi (haemolytica) serotype T3 is functionally and genetically homologous to that of Escherichia coli

A DNA fragment capable of complementing a temperature-sensitive mutation in the 3-deoxy-D- mannooctulosonic acid (Kdo) 8-phosphate synthase gene ( kdsA) of Salmonella enterica sv Typhimurium AG701i50 was cloned from the reference strain of Pasteurella trehalosi (haemolytica) serotype T3. Subcloning and DNA sequence analysis identified an open reading frame (ORF) of 854 nucleotides which could encode a protein of 31.0 kDa. Protein expression of …