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Journal article

Scanning Transmission X-Ray, Laser Scanning, and Transmission Electron Microscopy Mapping of the Exopolymeric Matrix of Microbial Biofilms

Abstract

Confocal laser scanning microscopy (CLSM), transmission electron microscopy (TEM), and soft X-ray scanning transmission X-ray microscopy (STXM) were used to map the distribution of macromolecular subcomponents (e.g., polysaccharides, proteins, lipids, and nucleic acids) of biofilm cells and matrix. The biofilms were developed from river water supplemented with methanol, and although they comprised a complex microbial community, the biofilms …

Authors

Lawrence JR; Swerhone GDW; Leppard GG; Araki T; Zhang X; West MM; Hitchcock AP

Journal

Applied and Environmental Microbiology, Vol. 69, No. 9, pp. 5543–5554

Publisher

American Society for Microbiology

Publication Date

9 2003

DOI

10.1128/aem.69.9.5543-5554.2003

ISSN

0099-2240