ChIP-on-chip to Identify Mutant p53 Targets

Chromatin immunoprecipitation (ChIP) followed by microarray hybridization (on-chip) is a technique well suited for a comprehensive analysis of transcription factor binding sites, histone modification patterns, and nucleosome occupancy. It can be restricted to a subset of genes or regions but also expanded up to a genome-wide range yielding insight into the functional elements of gene regulatory networks. Mutant p53 proteins have lost their …