Journal article
Real-time fluorogenic kinase assay using protein as substrate
Abstract
A real-time fluorogenic kinase assay using myelin basic protein (MBP) as a substrate is reported. MBP is part of a noncovalent complex with a negatively charged, dye-labeled lipopeptide, (N-heptadecanoyl)-K(dye2)-linker-EEIYGEF-amide. The complex is approximately 20 times less fluorescent than the free lipopeptide. The MBP-lipopeptide complex serves as a protein substrate for several Ser/Thr kinases. We infer that the observed fluorescence …
Authors
Sun H; Mitchell K; Lee L
Journal
Analytical Biochemistry, Vol. 406, No. 1, pp. 14–18
Publisher
Elsevier
Publication Date
11 2010
DOI
10.1016/j.ab.2010.06.046
ISSN
0003-2697