LivePyxel: accelerating image annotations with a Python-integrated webcam live streaming

LivePyxel is a pixel-level annotation editor designed for the development of image segmentation models. Inspired by popular graphics editors, it supports precise annotations through Bézier spline–based tools and live-feed webcam integration. The lack of flexible annotation tools has hindered the deployment of AI models in some scientific areas. Most existing image annotation software requires users to upload a precollected dataset, which limits support for on-demand pipelines and introduces unnecessary steps to acquire images. This constraint is particularly problematic in laboratory environments, where on-site data acquisition from instruments such as microscopes is increasingly common. In this work, we introduce LivePixel, a Python-based graphical user interface that integrates with imaging systems, such as webcams, microscopes, and others, to enable on-site image annotation. LivePyxel is designed to be easy to use through a simple interface that allows users to precisely delimit areas for annotation using tools commonly found in commercial graphics editing software. Of particular interest is the availability of Bézier splines and binary masks, and the software's capacity to work with non-destructive layers that enable high-performance editing. LivePyxel also integrates a wide compatibility across video devices, and it's optimized for object detection operations via the use of OpenCV in combination with high-performance libraries designed to handle matrix and linear algebra operations via Numpy effectively. LivePyxel facilitates seamless data collection and labeling, accelerating the development of AI models in experimental workflows. LivePyxel is freely available at https://github.com/UGarCil/LivePyxel.