The long-term goal of this work is to use glucose-stimulated zinc secretion (GSZS) MRI in men undergoing scans for prostate cancer. Given that dietary choices may affect an individual’s zinc content we examined how dietary zinc impacts prostate cancer detection via GSZS MRI in healthy and TRAMP mice. Mice were fed a low, normal, and high zinc content diet. GSZS was evaluated by MRI, tissue zinc content by synchrotron radiation X-ray fluorescence (SR-XRF), and zinc bioavailability by ICP-MS of serum. Results showed zinc deficiency caused systemic and organ-level dysregulation, weight loss, and altered zinc bioavailability. Healthy mice increased serum zinc, while TRAMP mice maintained low levels. SR-XRF indicated reduced prostate zinc in zinc-deficient diets for both WT and TRAMP mice. Normalized GSZS MRI data indicated stimulated zinc efflux increased with dietary zinc in healthy mice but not in TRAMP mice. The study underscores the potential role of dietary zinc in prostate cancer detection.