Autonomous Exponential Amplification via Rolling Circle–DNAzyme Feedback Programming

Rolling circle amplification (RCA) is a powerful isothermal strategy for nucleic acid detection, but its linear kinetics and dependence on externally supplied primers limit its sensitivity and programmability. Here, we report an exponential RCA (E-RCA) platform that integrates primer regeneration and signal amplification into a single DNA-encoded system. The design uses a circular DNA template encoding the I-R3 self-cleaving DNAzyme sequence; …