Efficient Migration Assay for Brain Tumor Stem Cells

A defining characteristic of brain tumor stem cells (BTSCs) is their ability to migrate and invade the brain parenchyma. This invasive behavior is particularly critical in understanding how BTSCs originating from primary tumors contribute to the formation of secondary brain tumor lesions. In this chapter, we present a streamlined and unbiased method to assess BTSC migration capacity. By leveraging real-time imaging with the Incucyte system, we provide a standardized and efficient approach for studying BTSC motility under controlled conditions.