Journal article
Direct Quantification of PEGylation for Intact Bioconjugates and Nanoparticles by the Colorimetric Barium/Iodide Assay
Abstract
Several methods are available to determine the average number of methoxy poly(ethylene glycol) (mPEG) chains grafted to a protein or peptide, referred to as the degree of PEGylation. Nevertheless, the development of simple, versatile, and multiplexable methods for determining PEGylation remains desirable. Childs and Kurfürst have respectfully reported the quantitative and qualitative use of a colored 'barium-iodide-PEG' complex for the …
Authors
Coutu K; Sangwa AN; Gaudreault N; Tayebi SS; Hoare T; Mhaskar P; Bertrand N; Greschner AA; Gauthier MA
Journal
Biomacromolecules, Vol. 26, No. 6, pp. 3689–3699
Publisher
American Chemical Society (ACS)
Publication Date
June 9, 2025
DOI
10.1021/acs.biomac.5c00274
ISSN
1525-7797