Electrochemical Studies of the Interaction of 2‐Nitroacridone with DNA and Determination of DNA

Abstract A new acridone derivate 2‐nitroacridone (NAD) was synthesized and a new method of electrochemical probe has been proposed for the determination of salmon sperm DNA based on its interaction with NAD. The electrochemical behavior of interaction of NAD with DNA was investigated on glassy carbon electrode (GCE). In the presence of DNA, the peak current of NAD decreases and the peak potential shifts to a more positive potential without appearance of a new peak. The binding ratio between NAD and salmon sperm DNA was calculated to be 2 : 1 and the binding constant was 3.19×10 5 L/mol. The decrease of the peak current (Δ I p ) of NAD was proportional to the concentration of DNA in the range from 1.55×10 −7 M to 2.02×10 −6 M with the detection limit of 3.10×10 −8 M, and DNA of synthetic sample was determined satisfactorily. Additionally, the binding mechanism was preliminarily discussed. The mode of interaction between NAD and DNA was found to be intercalation binding.