Introduction: Prior to the COVID-19 pandemic, physician burnout was endemic in North America. The pandemic placed an increase demand on physicians’ time both at work and home. We sought to identify the frequency of burnout in a large academic institution and its impact on clinical practice, non-clinical work, and homelife. Methods: All academic physicians and non-physician faculty members in the Department of Medicine (DOM) at McMaster University were invited to participate in an anonymous survey between January 22 and February 21, 2021. The survey elicited information on how clinical practice, work, and home life changed over the course of the pandemic. Responses to questions were captured on a 1-to-5 Likert scale. Descriptive statistics and the Mann Whitney U test were used for determining statistical significance (p< 0.05). The results were compared to the 2019 DOM survey which included a question on burnout.Results: Among 330-faculty, 76.7% completed the survey. The reported burnout was high at 75.9%, affecting women to a greater extent than men faculty (82.5% vs 70.4%, P< 0.01). Early career faculty also reported proportionally more burnout (83.5% versus 65.7%; p< 0.001). Medical-legal liability of phone-based care was a concern for 48% of physicians. The reported hours of work per day was significantly higher amongst women compared with men (80.4% vs 58.0%; p< 0.001). Loneliness (64.1% vs 51.4%; p< 0.05) and hours spent on caring for dependents (54.5% vs 31.1%, p< 0.01) were higher for women versus men. Both genders reported career fulfillment and research productivity were overall lower by 51.2% and 52.3% respectively. Conclusions: The COVID-19 pandemic has increased burnout amongst DOM academic faculty, and disproportionately affected women and early career faculty. A thoughtful systems level approach, with dedicated resources, is needed to address the impact of the COVID-19 pandemic, especially for women and early career faculty.