Effects of Processing at 45 C on Staining

Tissue processed at a constant temperature of 45 C including the use of paraffin wax with a melting point of 45 C displays staining characteristics that are sometimes reversed from those associated with the more usual processing schedules and wax with a melting point of 58-60 C. Staining with acid dyes, particularly in trichrome methods, are most susceptible to these changes. We suggest that this is directly related to dye molecular size and to …