Treatment of non-healing wounds has remained difficult, in spite of better understanding of pathophysiologic principles. Besides advances stemming from breakthroughs in recombinant growth factors and bioengineered skin, early data suggest the use of multipotent stem cells in order to accelerate wound healing. Until recently, research has been mostly focused on bone marrow-derived mesenchymal stem cells; however, other types of stem cells, including adipose-derived stem cells (ADSCs) and those derived from hair follicles gain more and more interest for potential application for the repair and regeneration of various damaged tissues. The aim of this chapter is to discuss the use and the potential of this novel technology.