Microsatellite isolation from four avian species comparing two isolation techniques

Abstract Microsatellites are continuing to increase in popularity for genetic studies. A major drawback, however, is they often need to be isolated de novo . The traditional method (shotgun cloning) generally results in thousands of recombinant clones, which are then screened by Southern hybridization. Results are typically around 2% positive for mammals, but less than 0.4% for birds. Shotgun cloning is therefore generally not an effective …