Journal article
Development and optimization of a Q-RT PCR method to quantify CYP19 mRNA expression in testis of male adult Xenopus laevis: Comparisons with aromatase enzyme activity
Abstract
Due to limitations of the currently used enzymatic assays, it is difficult to determine aromatase activity in testicular tissue of amphibians. Quantitative reverse transcription polymerase chain reaction (Q-RT PCR) is a sensitive and reliable technique to detect low amounts of mRNA for specific genes. This study was designed to develop and optimize a SYBR Green I-based Q-RT PCR method to quantify CYP19 mRNA in testicular tissue from male …
Authors
Park J-W; Hecker M; Murphy MB; Jones PD; Solomon KR; Van Der Kraak G; Carr JA; Smith EE; du Preez L; Kendall RJ
Journal
Comparative Biochemistry and Physiology Part B Biochemistry and Molecular Biology, Vol. 144, No. 1, pp. 18–28
Publisher
Elsevier
Publication Date
May 2006
DOI
10.1016/j.cbpb.2006.01.003
ISSN
1096-4959